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Exploring biomarkers in the overactive bladder: Alterations in miRNA levels of a panel of genes in patients with OAB
Author(s) -
Fırat Elif,
Aybek Zafer,
Akgün Şakir,
Küçüker Kürşat,
Akça Hakan,
Aybek Hülya
Publication year - 2019
Publication title -
neurourology and urodynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 90
eISSN - 1520-6777
pISSN - 0733-2467
DOI - 10.1002/nau.24065
Subject(s) - overactive bladder , microrna , medicine , receiver operating characteristic , diagnostic biomarker , pathophysiology , area under the curve , disease , pathogenesis , bioinformatics , diagnostic accuracy , gene , pathology , biology , genetics , alternative medicine
Aims It has been demonstrated that there are abundant stable microRNAs (miRNAs) in plasma, which is potentially disease‐specific. Adrenergic and muscarinic pathways play an important role in voiding physiology. Alterations in the levels of miRNAs are thought to influence the regulation of these pathways at the molecular level. The aim of this study was to investigate the relationship of miRNAs with overactive bladder pathogenesis and to provide a new perspective to treatment approaches. Methods This study included patients with an overactive bladder (OAB) diagnosis and a healthy control group. All patients completed a validated OAB‐V8 questionnaire. The relative expression levels of 12 miRNAs were examined in plasma by PCR. Receiver operating characteristic (ROC) curves were generated to evaluate the diagnostic qualification of miRNAs. Results The relative expression levels of let‐7b‐5p, miR‐92a‐3p, miR‐98‐5p, miR‐142‐3p, and miR‐200c‐3p were significantly upregulated and miR‐139‐5p was significantly downregulated in patients with OAB and no correlation was determined between the levels of miRNAs with OAB symptom score. Among the miRNAs, miR‐98‐5p provided the highest diagnostic accuracy alone (area under curve [AUC] = 0.79) in ROC analysis. The combination of miR‐98‐5p + miR‐139‐5p was seen to be a good indicator (AUC = 0.839). Conclusions These results suggest that alteration of the miRNA levels can be used as auxiliary parameters to explain the pathophysiology of OAB syndrome and could shed light on new treatment options.