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Diagnostic value of urinary CXCL10 as a biomarker for predicting Hunner type interstitial cystitis
Author(s) -
Niimi Aya,
Igawa Yasuhiko,
Aizawa Naoki,
Honma Toshiki,
Nomiya Akira,
Akiyama Yoshiyuki,
Kamei Jun,
Fujimura Tetsuya,
Fukuhara Hiroshi,
Homma Yukio
Publication year - 2018
Publication title -
neurourology and urodynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 90
eISSN - 1520-6777
pISSN - 0733-2467
DOI - 10.1002/nau.23431
Subject(s) - biomarker , medicine , urinary system , interstitial cystitis , urine , cxcl10 , urology , creatinine , urinary bladder , overactive bladder , gastroenterology , chemokine , pathology , inflammation , chemistry , biochemistry , alternative medicine
Aim To investigate the feasibility of chemokines and cytokines potentially elevated in the bladder tissue of Hunner type interstitial cystitis (HIC) as urinary markers for distinguishing HIC from non‐Hunner type interstitial cystitis (NHIC) Methods Urine specimens were collected from 41 HIC patients, 25 NHIC patients, and 31 healthy volunteers (control). The supernatants of urine specimens were subjected to ELISA kits for measurements of 10 cytokines and chemokines, whose gene expression was known to be elevated in HIC bladder tissue. Urinary levels normalized by urinary creatinine (Cr) concentration were compared among three groups. Efficiency in differentiating IC and IC subtypes was explored by ROC analysis. The correlation of marker levels with symptom severity, assessed by O'Leary‐Sant's symptom index (OSSI) and problem index (OSPI), was examined. Results The urinary levels of CXCL10 and NGF were significantly higher in HIC than NHIC. CXCL10 and NGF differentiated HIC against NHIC with AUC of 0.78 and 0.68, respectively. Combination of CXCL10 and NGF levels yielded an AUS of 0.81. The CXCL10 cut‐off of 53.2 pg/mg Cr had sensitivity of 46.1%, specificity of 93.7%, positive predictive value of 97.7%, and negative predictive value of 60.0%. The urinary level of other cytokines showed no significant difference between HIC and NHIC. Significant correlation with symptoms was detected for CXCL10 alone. Conclusion The results suggested that increased urinary level of CXCL10 combined with or without high NGF level could be a promising supplementary biomarker for differentiating HIC from NHIC with modest sensitivity and high specificity.