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Tadalafil, a phosphodiesterase type 5 inhibitor, improves bladder blood supply and restores the initial phase of lower urinary tract dysfunction in diabetic rats
Author(s) -
Gotoh Daisuke,
Torimoto Kazumasa,
Tatsumi Yoshihiro,
Hori Shunta,
Yamada Atsushi,
Miyake Makito,
Morizawa Yosuke,
Aoki Katsuya,
Tanaka Nobumichi,
Hirayama Akihide,
Fujimoto Kiyohide
Publication year - 2018
Publication title -
neurourology and urodynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 90
eISSN - 1520-6777
pISSN - 0733-2467
DOI - 10.1002/nau.23372
Subject(s) - medicine , tadalafil , urology , urinary system , diabetes mellitus , urination , endocrinology , cystometry , lower urinary tract symptoms , intraperitoneal injection , erectile dysfunction , prostate , cancer
Aims To investigate the effect of tadalafil on bladder blood flow and lower urinary tract function in a rat model of diabetes. Materials and Methods We studied female Sprague‐Dawley rats and induced diabetes in some using a single intraperitoneal injection of streptozotocin. We divided the rats into nondiabetes (ND), diabetes (D), and diabetes with tadalafil (DT) groups. The rats were raised for an additional 7 weeks after diabetes induction. The DT group received oral tadalafil (2 mg/kg/day) for 7 days before the experiments. At 7 weeks after diabetes induction, we performed cystometry, resected the bladders for immunohistochemistry (hypoxia‐inducible factor‐1α [HIF‐1α] and 8‐oxo‐2′‐deoxyguanosine [8‐OHdG] staining), and measured bladder blood supply using a laser blood flow meter. Results The opening pressure, when the urethra opens and urine flow starts, was significantly lower in the DT group than in the D group (24.9 ± 5.9 vs 43.6 ± 12.3 cmH 2 O). The inter‐contraction interval was significantly longer in the D group than in the ND and DT groups (1566.2 ± 168.7 vs 702.9 ± 165.2 and 787.4 ± 148.8 s). Immunohistochemistry showed positive staining of the urothelial layer for both HIF‐1α and 8‐OHdG in the D group, but not in the ND or DT groups. Bladder blood flow was significantly lower in the D group than in the ND or DT groups. Conclusions Tadalafil improves bladder blood supply and lower urinary tract function in diabetic rats. Tadalafil may be a promising drug that restores lower urinary tract dysfunction in the early phase of diabetes.