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Combination histamine and serotonin treatment after simulated childbirth injury improves stress urinary incontinence
Author(s) -
Song QiXiang,
Balog Brian M.,
Lin Dan Li,
Jiang HaiHong,
Gurel Volkan,
Spaulding James,
Tobacyk Julia,
McMichael John,
Damaser Margot S.
Publication year - 2016
Publication title -
neurourology and urodynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 90
eISSN - 1520-6777
pISSN - 0733-2467
DOI - 10.1002/nau.22793
Subject(s) - medicine , histamine , serotonin , urethra , urethral sphincter , urology , urinary incontinence , distension , urination , saline , anesthesia , urinary system , receptor
Aims Histamine and serotonin‐related pharmaceuticals have the potential to modulate micturition and continence. The aim of this study was to determine if treatment with histamine and/or serotonin improves stress urinary incontinence (SUI) in female rats. Methods Twenty‐six age‐matched female rats underwent pudendal nerve crush and vaginal distension (PNC + VD), to produce SUI. One week after injury, rats were treated subcutaneously with saline, histamine (1.1 μg), serotonin (2μg), or the combination of both twice daily for another week. A sham injured group received sham PNC + VD and were treated with saline (n = 7). Leak point pressure (LPP) testing with simultaneous external urethral sphincter (EUS) electromyography (EMG) was conducted 2 weeks after injury. The urethra was harvested for qualitative and quantitative histology. Data were analyzed with a one‐way ANOVA and Student‐Newman‐Keuls posthoc test with P < 0.05 indicating statistically significant differences between groups. Results Combination treatment significantly increased LPP after PNC + VD compared to injured sham treatment and treatment with either histamine or serotonin alone. Compared to injured sham treated rats, all three treatments significantly increased EUS EMG amplitude at both baseline and peak pressure and EUS EMG firing rate at peak pressure during LPP testing. There were more consistent urethral striated muscle fibers and thicker smooth and striated muscle with combination and histamine treatment. There was a statistically significant shift to a greater proportion of thicker collagen fibers in the urethra in serotonin and combination treated rats compared with injured sham treated rats. Conclusions Combination treatment was the most effective and may provide an effective therapy for SUI. Neurourol. Urodynam. 35:703–710, 2016 . © 2015 Wiley Periodicals, Inc.