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Stimulation of skin menthol receptors stimulates detrusor activity in conscious rats
Author(s) -
Chen Zhong,
Ishizuka Osamu,
Imamura Tetsuya,
Aizawa Naoki,
Kurizaki Yoshiki,
Igawa Yasuhiko,
Nishizawa Osamu,
Andersson KarlErik
Publication year - 2010
Publication title -
neurourology and urodynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 90
eISSN - 1520-6777
pISSN - 0733-2467
DOI - 10.1002/nau.20774
Subject(s) - trpm8 , medicine , menthol , stimulation , cystometry , receptor , saline , transient receptor potential channel , urination , endocrinology , anesthesia , pharmacology , urinary bladder , urinary system , chemistry , trpv1 , organic chemistry
Aims The Transient Receptor Potential Melastatin (TRPM)8 channel, activated by menthol and cool temperatures, has been proposed to be a cool receptor involved in mediation of the bladder response in the diagnostic ice water test. We examined the expression of TRPM8 in the rat skin and investigated if stimulation of skin menthol receptors by menthol can induce detrusor activity in conscious rats. Methods Immunofluorescence staining for TRPM8 was performed in samples of the skin from the leg and back of the rats. Continuous cystometry was performed in conscious animals. Voiding interval (VI), micturition volume (MV), and bladder capacity (BC) were evaluated before and after spraying menthol solution to the skin of the leg and back of the rats. Results Immunohistochemical analysis revealed that TRPM8 was expressed in the investigated tissues. The expression of TRPM8‐positive receptors was not significantly different between the leg and back skin. Saline caused no significant change in cystometric parameters. After spraying menthol solution (50% and 99% to the skin of the leg, and 99% to the back skin), VI, MV, and BC decreased significantly. Conclusions TRPM8 is expressed in rat skin. Spraying menthol solution onto the skin‐induced detrusor activity, an effect we suggest is mediated by stimulation of TRPM8 receptors. Neurourol. Urodynam. 29:506–511, 2010. © 2009 Wiley‐Liss, Inc.