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The extracorporeal perfusion of the female pig detrusor as an experimental model for the study of bladder contractility
Author(s) -
Dittrich Ralf,
Beckmann Matthias W.,
Maake Caroline,
Oppelt Patricia G.,
Mueller Andreas,
Mueller Siegfried,
Hoffmann Inge,
Naumann Gert,
Koelbl Heinz,
Maltaris Theodoros
Publication year - 2007
Publication title -
neurourology and urodynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 90
eISSN - 1520-6777
pISSN - 0733-2467
DOI - 10.1002/nau.20443
Subject(s) - carbachol , contractility , perfusion , medicine , urinary bladder , ischemia , extracorporeal , urology , detrusor muscle , contraction (grammar) , bicarbonate , anesthesia , stimulation
Aims The objective of the study was to establish an experimental model for the extracorporeal perfusion of the pig detrusor. In order to validate this model we examined some biochemical parameters and determined the effect of carbachol on the contractility of perfused female pig bladders. Methods Twenty‐six pig bladders were perfused with Krebs–Ringer bicarbonate‐glucose buffer for a period up to 5 hr with the aim to preserve a viable organ, which would be responsive to contraction‐inducing agents. The intravesical pressure of the bladder as well as the intraarterial pressure of the vesical arteries were recorded before and after administration of carbachol. Results The perfusate pH, lactate, partial carbon dioxide tension, and the ATP content in the perfused tissue, all indicators of tissue ischemia or cell necrosis, showed a good preservation of the organ for up to 5 hr. Carbachol was able to induce contractions of the prefilled bladder with a complete draining of the bladder throughout the whole perfusion period. Conclusions We could demonstrate that this perfusion system was able to preserve the pig bladder in a functional condition, appropriate for the study of physiological questions. Neurourol. Urodynam. 26:1024–1029, 2007. © 2007 Wiley‐Liss, Inc.