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Novel biomarkers of bladder decompensation after partial bladder obstruction
Author(s) -
Guven Ahmet,
Kalorin Carmin,
Onal Bulent,
Whitbeck Catherine,
Chichester Paul,
Kogan Barry A.,
Levin Robert M.,
Mannikarottu Anita
Publication year - 2007
Publication title -
neurourology and urodynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 90
eISSN - 1520-6777
pISSN - 0733-2467
DOI - 10.1002/nau.20433
Subject(s) - decompensation , medicine , urology , contractility , bladder outlet obstruction , cystometry , superoxide dismutase , urinary bladder , endocrinology , prostate , oxidative stress , cancer
Aims Partial bladder outlet obstruction (PBOO) results in marked contractile, biochemical, and histological alterations in the bladder. Our aim was to determine the time course of progressive PBOO in the rabbit and to find parameters that marked the shift to decompensation. Materials and Methods Twenty‐four rabbits were subjected to 1, 2, 4, and 8 weeks of PBOO. Sham operated rabbits served as controls. At each time period, cystometry was performed and individual bladder strips were used for contractility studies. Full‐thickness sections of bladder body from each rabbit were fixed in formalin and used to determine the vascular density and nerve density. The balance of the bladder body was separated between muscle and mucosa and was analyzed for superoxide dismutase (SOD) and catalase (CAT) activities. Results Bladder weight increased progressively and all contractile responses were reduced significantly over the course of obstruction. Markedly increased bladder weight and very large bladder volumes indicated decompensation. Nerve density was marked decreased in decompensated bladders. Similarly, SOD activity in muscle decreased progressively and was markedly lower in decompensated bladders. Although CAT activity of the muscle increased after 2–4 weeks of obstruction, it decreased markedly in decompensated bladders. Conclusion This study shows that prolonged PBOO causes progressive deterioration in the rabbit bladder with decompensation after 8 weeks. Markedly decreased nerve density and severely reduced SOD and CAT activities are associated with the shift from compensated to decompensated function of the bladder. They may be excellent biomarkers of decompensation. Neurourol. Urodynam. 26:1036–1042, 2007. © 2007 Wiley‐Liss, Inc.