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Effects of local anesthetics on human bladder contractility
Author(s) -
Oh SeungJune,
Paick Sung Hyun,
Lim Dae Jung,
Lee Eunsik,
Lee Sang Eun
Publication year - 2005
Publication title -
neurourology and urodynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 90
eISSN - 1520-6777
pISSN - 0733-2467
DOI - 10.1002/nau.20113
Subject(s) - tetracaine , local anesthetic , bupivacaine , contraction (grammar) , contractility , lidocaine , medicine , anesthesia , ropivacaine , isometric exercise , stimulation , procaine , muscle contraction , tonic (physiology) , pharmacology , endocrinology
Aims We investigated the invitro effects of local anesthetics on the contractility of the human bladder. Methods By measuring the invitro isometric contractions of human bladder strips, we determined the effects of tetracaine, bupivacaine, lidocaine, and ropivacaine on the basal spontaneous contractions and contractions induced by various stimuli, namely, KCl (60 mM), carbachol (CCh), and electrical field stimulation (EFS). The effect of local anesthetic agents on Ca 2+ ‐independent sustained tonic contraction (SuTC) of the detrusor was also investigated. Results Local anesthetics increased phasic and tonic spontaneous contractile activity dose dependently in the concentration range 1–500 μM, but abolished phasic activity at higher concentrations. Local anesthetic agents inhibited nerve‐mediated contraction (EFS, 0.8 msec) in a concentration‐dependent manner (ropivacaine > tetracaine = bupivacaine > lidocaine), and inhibited non‐nerve mediated contractions induced by KCl, long pulse EFS (direct muscle stimulation, 100 msec), and CCh. Inhibitory potency on non‐nerve mediated contraction was for long pulse EFS: ropivacaine = tetracaine > bupivacaine = lidocaine and for KCl‐ and CCh‐induced contractions: ropivacaine > tetracaine > bupivacaine = lidocaine. Higher concentrations of local anesthetics were needed to inhibit non‐nerve‐mediated bladder contraction than nerve‐mediated contraction. SuTC was suppressed by all local anesthetics concentration dependently. Conclusions Our study demonstrates that local anesthetics have inhibitory effects on the contraction of human bladder as induced by different stimulants and concentrations. Their effects and differences suggest that they may be considered potentially useful as diagnostic and therapeutic agents for bladder dysfunction. © 2005 Wiley‐Liss, Inc.