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In vitro effects of intravesical dimethylsulfoxide
Author(s) -
Freedman Alan I.,
Wein Alan J.,
Whitmore Kristene,
Levin R. M.
Publication year - 1989
Publication title -
neurourology and urodynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 90
eISSN - 1520-6777
pISSN - 0733-2467
DOI - 10.1002/nau.1930080315
Subject(s) - bethanechol , cystometry , medicine , stimulation , urology , interstitial cystitis , in vitro , urinary bladder , anesthesia , urinary system , chemistry , biochemistry , receptor , muscarinic acetylcholine receptor
The mainstay of therapy for interstitial cystitis (IC) is intravesical instillation of dimethylsulfoxide (DMSO). Although numerous clinical studies have documented symptomatic improvement in up to 70% of patients, the mechanism of action, and effect on objective parameters of bladder function have not been adequately studied. In this study we have used the in vitro whole bladder model to determine the effect of DMSO on several parameters of bladder function, including in vitro cystometry, pressure generation, and ability to empty in response to both field stimulation and bethanechol. All parameters measured were significantly ( P < 0.05) reduced. Bladder capacity was reduced by a mean of 18.71%. Compliance was markedly decreased after instillation of DMSO. The magnitude of pressure generated in response to field stimulation was decreased from a mean of 8.93 cm H 2 O to a mean of 5.55 cm H 2 O. In response to bethanechol, the magnitude of pressure generation was decreased from a mean of 9.85 cm H 2 O to a mean of 7.07 cm H 2 O after treatment with DMSO. The rate of pressure generation in response to field stimulation was substantially reduced by 46.6%. In response to field stimulation and bethanechol, control bladders emptied 86% and 92%, respectively. After treatment with DMSO, these values were reduced by an average of 33.24% and 14.92%, respectively. This in vitro study has shown that DMSO has a negative effect on multiple parameters of bladder function, including compliance, capacity, and contractile and functional responses to field stimulation and bethanechol.