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Quantitative study in vitro of the spontaneous contractions of pig detrusor muscle and their frequency‐selective inhibition by drugs
Author(s) -
Hak Jaap,
Griffiths Derek J.,
van Mastrigt Ron,
Koopal Bouwe,
Klück Paul
Publication year - 1988
Publication title -
neurourology and urodynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 90
eISSN - 1520-6777
pISSN - 0733-2467
DOI - 10.1002/nau.1930070509
Subject(s) - hexamethonium , atropine , low frequency , bromide , detrusor muscle , in vitro , medicine , muscle contraction , endocrinology , smooth muscle , chemistry , biochemistry , physics , organic chemistry , astronomy
The amplitudes of the various frequency components present in the spontaneous contractions of pig detrusor muscle in vitro have been analysed and their responses to drugs examined. For strips taken from the ventral wall of the bladder the spontaneous contractions typically show one or both of two main frequency components near 0.02 Hz (1 per 50 s) and 0.05 Hz (1 per 20 s), respectively. The relative prominence of the two components differs from strip to strip. Spontaneous activity in strips showing mainly the lower frequency is strongly inhibited by oxyphenonium bromide (3 × 10 −5 M). Activity in strips showing mainly the higher frequency is almost unaffected. Atropine sulphate (10 −6 M) has no consistent effect on either of the two frequency components. Hexamethonium bromide (10 −5 ) selectively inhibits the low‐frequency component. Histological examination shows that the strips contain ganglion cells and suggests that those with mainly low‐frequency activity contain many more such cells than those with higher‐frequency activity. This observation is consistent with the observed frequency‐selective effect of hexamethonium bromide.