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Evidence against the presence of spare muscarinic receptors in the rabbit urinary bladder
Author(s) -
Levin Robert M.,
High Janice,
Wein Alan J.
Publication year - 1983
Publication title -
neurourology and urodynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 90
eISSN - 1520-6777
pISSN - 0733-2467
DOI - 10.1002/nau.1930020408
Subject(s) - muscarinic acetylcholine receptor , bethanechol , urinary bladder , medicine , endocrinology , receptor , stimulation , urinary system
Abstract In a recent study by Anderson and Marks [1982], indirect evidence was presented for the existence of “150‐fold muscarinic receptor excess” in the rabbit urinary bladder. This conclusion was based on the quantitative comparison of the ability of carbamylcholine to both directly contract bladder strips and to inhibit (quinuclidinyl benzilate) binding. In order to investigate the presence of “spare receptors” in the bladder directly, we have determined the effect of benzilylcholine mustard (a noncompetitive cholinergic inhibitor) on both bethanechol stimulation of muscle‐strip contraction and on [ 3 H]QNB binding (muscarinic receptor density). The results of these studies indicate that there is no significant “muscarinic receptor excess” in the rabbit urinary bladder.