Premium
Excretion of collagen derived peptides is increased in women with stress urinary incontinence
Author(s) -
Kushner Leslie,
Mathrubutham Mahesh,
Burney Tracey,
Greenwald Robert,
Badlani Gopal
Publication year - 2004
Publication title -
neurourology and urodynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 90
eISSN - 1520-6777
pISSN - 0733-2467
DOI - 10.1002/nau.10174
Subject(s) - medicine , urinary incontinence , urology , excretion , urinary system , endocrinology , physiology
Aims The objective of this study was to demonstrate that weakened pelvic floor support of the lower genitourinary tract in women with stress urinary incontinence (SUI) is due to increased collagenolysis. When fibrillar collagen is degraded, pyridinium (PYD) crosslinks are released and excreted in the urine. Degradation of collagen also results in peptide fragments of various lengths which are excreted in the urine. Degradation of mature fibrillar collagen and collagen which has not been crosslinked can be assessed independently by measurement of both PYD and collagen‐derived peptides in the urine. Methods Twenty‐four hour urine collections were obtained from women with SUI (n = 23) and women without urinary incontinence (n = 39). Urinary PYD concentration was assayed by ELISA. The urinary concentration of helical peptide α1 (I) 620–633 fragments derived from collagen was assayed by competitive enzyme immunoassay. Values were normalized to creatinine. Results The mean urine PYD concentration for women with SUI (110.8 ± 19.7 nM/mM creatinine) was not significantly different than that for women without SUI (85.2 ± 13.7 nM/mM creatinine). The mean urine concentration of helical peptide α1 (I) 620–633 for women with SUI (0.80 ± 0.13 μg/mg creatinine) was significantly ( P < 0.02) higher than that for women without SUI (0.49 ± 0.06 μg/mg creatinine). Conclusions These data suggest that collagenolytic activity in women with SUI is elevated compared to continent controls, as measured by urinary helical peptide α1 (I) 620–633 excretion. The lack of difference in urinary PYD excretion between the two populations suggests that the increased collagenolytic activity in women with SUI, compared to continent controls, is restricted to uncrosslinked collagen. Neurourol. Urodynam. 23:198–203, 2004. © 2004 Wiley‐Liss, Inc.