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Acute Effects of Temperature, pH, and Suspended Solids on Antioxidant and Digestive Enzyme Activities and Stress‐Response Gene Expressions in the Boring Giant Clam Tridacna crocea
Author(s) -
Ma Shaobo,
Qin Geng,
Zhang Bo,
Li Chunyan,
Fang Shaobin,
Yin Jianping
Publication year - 2021
Publication title -
north american journal of aquaculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 41
eISSN - 1548-8454
pISSN - 1522-2055
DOI - 10.1002/naaq.10178
Subject(s) - biology , hepatopancreas , amylase , digestive enzyme , lipase , gill , biochemistry , zoology , food science , enzyme , fishery , fish <actinopterygii>
Abstract The present study investigated the acute effects of three key environmental factors (temperature, pH, and suspended solids) on the physiology of the boring giant clam Tridacna crocea . Single‐factor experiments for a duration time of 10 h showed that high thermal stress (25 [control], 28, and 30°C), water acidification (pH 8.0 [control], 7.5, and 7.0), and suspended solids (no sands [control], or coral sand with grain size either 0.1 or 0.5 mm) all increased the mRNA expressions of heat‐shock‐protein genes and apoptosis‐related genes, respectively, in the gills of giant clams. Both water acidification and suspended solids also up‐regulated lactic acid dehydrogenase and glutamic‐pyruvate transaminase enzyme activities. There was no significant variation among the different treatment conditions in the digestive enzyme (trypsin, lipase, and amylase) activities in the hepatopancreas of giant clams, except that significant higher lipase activities were observed in those that were exposed to 28°C and pH 7.0. The present results imply that temperature, pH, and suspended solids are important environmental conditions that affect the physiology of giant clams during large‐scale artificial breeding.