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Evaluating Temperature and Pressure Shock to Create Gynogenetic Cloned Progeny in Southern Flounder
Author(s) -
Silvy Elizabeth H.,
Sink Todd D.
Publication year - 2020
Publication title -
north american journal of aquaculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 41
eISSN - 1548-8454
pISSN - 1522-2055
DOI - 10.1002/naaq.10160
Subject(s) - biology , milt , flounder , shock (circulatory) , andrology , sperm , offspring , ploidy , zoology , aquaculture , fishery , fish <actinopterygii> , genetics , medicine , gene , pregnancy
Abstract To address the needs of the growing aquaculture industry, we evaluated methodologies for creating gynogenetic female clones of Southern Flounder Paralichthys lethostigma . The primary objective was to determine which treatment to stop ejection of the second polar body, pressure or cold shock, would induce diploid genesis in ova that were activated with irradiated sperm and produce more viable offspring in Southern Flounder. The cold‐shock and pressure‐shock treatments with irradiated milt had similar egg‐activation rates and embryonic development rates of 1.3% (SE = 0.9) and 5.8% (SE = 2.7), respectively. However, pressure‐shock represents the most practical method for creating gynogenetic clones of the female.