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Detection of HTLV‐I provirus by in situ polymerase chain reaction in mononuclear inflammatory cells in skeletal muscle of viral carriers with polymyositis
Author(s) -
Higuchi I.,
Hashimoto K.,
Kashio N.,
Izumo S.,
Inose M.,
Izumi K.,
Ohkubo R.,
Nakagawa M.,
Arimura K.,
Osame M.
Publication year - 1995
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880180809
Subject(s) - polymyositis , provirus , polymerase chain reaction , peripheral blood mononuclear cell , virology , virus , skeletal muscle , medicine , immunology , biology , gene , in vitro , genome , biochemistry
Abstract We tested for HTLV‐I proviral DNA in skeletal muscle from patients with polymyositis infected with HTLV‐I using the in situ polymerase chain reaction. We found the HTLV‐I provirus in some of the CD4‐positive cells in HTLV‐I‐positive polymyositis cases but not in HTLV‐I‐negative polymyositis ones. We could not detect HTLV‐I within the muscle fibers. We suggest that HTLV‐I‐associated polymyositis is not due to direct, persistent infection of the muscle fiber by the virus, but to a T‐cell‐mediated immunological process triggered by the HTLV‐I‐infected cells. © 1995 John Wiley & Sons, Inc.