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Putative effects of nerve extract on carbonic anhydrase III expression in rat muscles
Author(s) -
Milot Julie,
CǒTé Claude H.,
Tremblay Roland R.
Publication year - 1994
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880171212
Subject(s) - denervation , carbonic anhydrase , endocrinology , medicine , chemistry , sciatic nerve , neurotrophin , saline , neurotrophic factors , skeletal muscle , soleus muscle , gene isoform , messenger rna , extensor digitorum longus muscle , enzyme , biology , biochemistry , receptor , gene
Carbonic anhydrase III (CA III), the predominant CA isoform in skeletal muscle is very sensitive to neuronal influences. We aimed to determine whether CA III expression could be influenced by neurotrophic factor(s) present in sciatic nerve extract (SNE). Intact muscles were thus compared with denervated soleus (SOL), extensor digitorum longus (EDL), and tibialis anterior (TA) muscles injected daily for 7 days with saline solution (SS) or with SNE. CA III activity was significantly increased in SS‐treated EDL and TA muscles compared to control (CTR), while SNE injections partially prevented this increase. There was no significant difference for CA III activity in the SOL between CTR, SS, and SNE groups. The CA III mRNA increase observed in response to denervation was reduced by 40% in SNE‐treated EDL and TA muscles. While SOL CA III mRNA level was not affected by denervation, a 52% decrease was observed with SNE. We concluded that neuronal modulation of CA III expression in type II fibers may involve a neurotrophic component. © 1994 John Wiley & Sons, Inc.