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Time course of changes in plasma membrane permeability in the dystrophin‐deficient mdx mouse
Author(s) -
McArdle Anne,
Edwards Richard H. T.,
Jackson Malcolm J.
Publication year - 1994
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880171206
Subject(s) - dystrophin , mdx mouse , extracellular , creatine kinase , endocrinology , medicine , chemistry , cytosol , duchenne muscular dystrophy , biology , biochemistry , enzyme
Control C57BI/10 and mutant, dystrophin‐deficient mdx mice of different ages were used to study the permeability of the plasma membrane to cytosolic components, to a vital stain (procion orange) and to extra‐cellular 45 calcium. Prenecrotic, 14 ± 2‐day‐old mdx mice had normal serum activities of creatine kinase (CK) and pyruvate kinase (PK). Muscles from these animals also had no increased permeability to procion orange or extracellular 45 calcium. Serum activities of CK and PK had risen acutely in the 21‐day‐old mdx mouse compared with control and remained elevated up to 6 months of age. The influx of procion orange and 45 calcium content were abnormally elevated in the 40 ± 4‐day‐old mdx mouse. These data provide no evidence for an increase in muscle plasma membrane permeability as a primary pathogenic effect of a lack of dystrophin, but results suggest that some factor expressed or deexpressed during mouse development may be necessary for the full expression of the dystrophic process. © 1994 John Wiley & Sons, Inc.