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Expression of myoglobin gene in skeletal muscle of patients with neuromuscular diseases
Author(s) -
Kawai Hisaomi,
Naruo Takako,
Yoneda Kenji,
Masuda Kenjiro,
Mitsui Takao,
Nishida Yoshihiko
Publication year - 1994
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880170703
Subject(s) - myoglobin , polymyositis , amyotrophic lateral sclerosis , duchenne muscular dystrophy , skeletal muscle , messenger rna , medicine , muscular dystrophy , endocrinology , gene expression , radioimmunoassay , chemistry , gene , biochemistry , disease
Expression of the myoglobin (Mb) gene in skeletal muscle was studied in patients with Duchenne muscular dystrophy (DMD), polymyositis (PM), or amyotrophic lateral sclerosis (ALS) by measuring Mb concentration by radioimmunoassay and Mb messenger ribonucleic acid (RNA) (MbmRNA) levels by Northern blot analysis. Mb concentrations in the muscle cells (Mb/noncollagenous protein) were decreased in patients with DMD, PM, or ALS. However, while Mb concentrations per MbmRNA content (Mb/MbmRNA) were decreased in DMD and PM patients, these values were normal in ALS patients. These results suggest that Mb synthesis is increased in muscles of DMD and PM patients, but is not sufficient to compensate for the excessive loss of Mb from the affected muscles, and that the synthesis is decreased in the muscles of ALS patients. © 1994 John Wiley & Sons, Inc.