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Acute conduction block in vitro following exposure to antiganglioside sera
Author(s) -
Arasaki Keisuke,
Kusunoki Susumu,
Kudo Norio,
Kanazawa Ichiro
Publication year - 1993
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880160603
Subject(s) - sciatic nerve , in vitro , nerve conduction velocity , pathogenesis , chemistry , compound muscle action potential , nerve fiber , antibody , anatomy , nerve conduction , electrophysiology , pathology , medicine , immunology , biochemistry
We studied the role of antiganglioside antibodies in the pathogenesis of autoimmune neuropathies using an in vitro preparation of a rat sciatic nerve. Human and rabbit sera with high titers of the antibodies were applied to a restricted segment of the sciatic nerve mounted in a recording chamber, and the compound nerve action potentials of the myelinated and unmyelinated fibers were observed. Myelinated fiber conduction became blocked at the segment within a few hours, whereas the unmyelinated fiber conduction remained unchanged. These results suggest that antiganglioside sera directly produce an acute conduction block only in myelinated nerve fibers and that this in vitro model is useful for studying the ionic mechanism by which the acute conduction block occurs. © 1993 John Wiley & Sons, Inc.