Increasing the proliferative capacity of muscular dystrophy myoblasts
Author(s) -
Yang Jie,
Seelig Miriam,
Rayner Sylvia,
Bredesen Dale E.
Publication year - 1992
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880150813
Subject(s) - muscular dystrophy , duchenne muscular dystrophy , myocyte , dystrophin , mdx mouse , retrovirus , biology , immortalised cell line , cell culture , microbiology and biotechnology , regeneration (biology) , recombinant dna , virology , immunology , genetics , gene
Abstract Temperature‐sensitive immortalized neural cells may be utilized to produce genetically engineered neural transplants. We have used a similar approach with mdx myoblasts. Control and mdx myoblasts were immortalized with a recombinant retrovirus that effects the expression of a temperaturesensitive simian virus 40 large T antigen. The resultant cells divide indefinitely at 34°C, but differentiate at 38°C, both morphologically and immunocytochemically. Polymerase chain reaction analysis of RNA confirmed the presence of the dystrophin point mutation in the mdx cells and its absence in the control cells. A similar approach may be useful for the proliferation, modification, and reimplantation of autologous cells from patients with degenerative and dystrophic disorders such as Duchenne muscular dystrophy. © 1992 John Wiley & Sons, Inc.
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