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Leakage of carbonic anhydrase III from normal and denervated rat skeletal muscle following contractile activity
Author(s) -
Ashmark Håkan,
Wistrand Per J.
Publication year - 1992
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880150602
Subject(s) - microdialysis , skeletal muscle , tibialis anterior muscle , in vivo , chemistry , muscle contraction , carbonic anhydrase , medicine , stimulation , contraction (grammar) , extracellular , anatomy , endocrinology , biology , biochemistry , enzyme , microbiology and biotechnology
Skeletal muscle extracellular carbonic anhydrase III was investigated in anesthetized rats by a microdialysis technique. A small dialysis probe was inserted into the tibialis anterior (TA) muscle and perfused continuously. Perfusates were collected before and during muscle contraction, induced by electrical stimulation of the muscle or of the sciatic nerve. In the perfusate of resting normal and denervated muscle, the concentration of CA III was 10 to 12 ng/mL, as measured by a radiommunosorbent technique. During contractile acitvity, the concentrations of CA III increased markedly in hte normal and denervated muscle. A TA muscle suspended in physiological saline behaved similarly, even though the leakage before and during contrafction was higher than in vivo. The results show that skeletal muscle leaks CA III both in vivo and in vitro, a leakage which was markedly increased by contractile activity. The microdialysis technique should also be useful in humans to study the efflux of various proteins from different kinds of diseased or fatigued muscles.