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Insulin effects in denervated and non‐weight‐bearing rat soleus muscle
Author(s) -
Tischler Marc E.,
Satarug Soisungwan,
Eisenfeld Steven H.,
Henriksen Erik J.,
Rosenberg Sara B.
Publication year - 1990
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880130706
Subject(s) - denervation , medicine , endocrinology , insulin , soleus muscle , protein degradation , stimulation , muscle atrophy , atrophy , protein turnover , biology , chemistry , skeletal muscle , protein biosynthesis , biochemistry
Previous reports indicated that glucose uptake in denervated muscle is resistant to insulin, while in non‐weight‐bearing (unweighted) muscle this effect of insuling is enhanced. To extend the comparison of these differences, insulin effects on amino acid uptake and protein metabolism were studied in soleus muscles subjected to denervation or unweighting. Denervated muscle showed insulin resistance of both 2‐deoxy[1,2‐ 3 H] glucose and ‐[methyl‐ 3 H]aminoisobutyric acid uptake whereas unweighted muscle showed an increased or normal response, respectively. Atrophy was greater in denervated than in unweighted muscle, apparently due to faster protein degradation by insulin was generally less in denervated than in unweighted muscle, apparently due to faster protein degradation. The stimulation of protein synthesis and the inhibition of protein degradation by insulin was generally less in denervated than in unweighted muscle. Since metabolic measurements in denervated‐unweighted muscles did not differ from those in denervated‐weight‐bearing muscles, effects of denervation must be independent of leg posture.