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Changes in skeletal muscle gene transcription induced by chronic stimulation
Author(s) -
Brownson Carol,
Isenberg Harvey,
Brown Wendy,
Salmons Stanley,
Edwards Yvonne
Publication year - 1988
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880111113
Subject(s) - skeletal muscle , stimulation , gene , transcription (linguistics) , medicine , biology , endocrinology , anatomy , genetics , linguistics , philosophy
The mRNA products of four genes, carbonic anhydrase III (CAIII), fast myosin heavy chain (MHCf), actin and glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH), were assayed by Northern and slot‐blot analysis in rabbit tibialis anterior and extensor digitorum longus muscles that were subjected to electrical stimulation for periods of up to 21 days. Marked changes in mRNA levels were seen for all four genes. The mRNA for CAIII, which is specific for type 1 fibers, rose significantly, whereas the MHCf mRNA fell markedly in the stimulated muscles. Changes in GAPDH mRNA were consistent with a reduced dependence on anaerobic glycolysis as an energy source. Actin mRNA levels were noticeably depressed in the early stages of stimulation. Thus for several classes of muscle protein, the response to chronic low‐frequency stimulation appears to involve changes at the level of gene transcription.