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Carnitine stimulation of pyruvate dehydrogenase complex (PDHC) in isolated human skeletal muscle mitochondria
Author(s) -
Uziel Graziella,
Garavaglia Barbara,
Di Donato Stefano
Publication year - 1988
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880110708
Subject(s) - pyruvate dehydrogenase complex , acetylcarnitine , carnitine , mitochondrion , pyruvate decarboxylation , skeletal muscle , stimulation , endocrinology , medicine , oxoglutarate dehydrogenase complex , pyruvate dehydrogenase kinase , chemistry , biochemistry , biology , pyruvate dehydrogenase phosphatase , enzyme
L‐carnitine stimulated CO 2 production from 1‐ 14 C pyruvate in mitochondria from human skeletal muscle nearly twofold. A comparable increase in the pyruvate dehydrogenase complex (PDHC) activity was seen. Moreover, in the presence of L‐carnitine and at pyruvate concentration greater than 0.25 m M , this effect was associated with a marked increase of acetylcarnitine synthesis. Deoxycarnitine, an inhibitor of carnitine acetyltransfersase (CAT), partially reversed the effect of carnitine on PDHC activity. The stimulatory effect of carnitine on PDHC activity in human mitochondria is mediated by the modulation of the intramitochondrial acetylCoA/CoASH ratio.