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Schwann cell‐conditioned medium supports neurite outgrowth and survival of spinal cord neurons in culture
Author(s) -
Bosch E. Peter,
Assouline Jose G.,
Pantazis Nicholas J.,
Lim Ramon Lim
Publication year - 1988
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880110408
Subject(s) - neurite , spinal cord , gdf7 , schwann cell , neurofilament , neurotrophic factors , in vitro , immunofluorescence , microbiology and biotechnology , biology , cell culture , neuroscience , neurotrophin , immunohistochemistry , anatomy , immunology , antibody , embryonic stem cell , biochemistry , receptor , genetics , gene
The effect of Schwann cell‐conditioned medium (SCM) on the development in vitro of spinal cord neurons was studied. Spinal cord neurons from 18‐day‐old rat embryos were cultured in serum‐free conditioned medium obtained from confluent rat Schwann cells. In cultures fed SCM, the cells developed typical neuronal morphology and were identified by indirect immunofluorescence using a monoclonal antibody to neurofilament protein. SCM stimulated neurite outgrowth and supported survival of spinal cord neurons. Preliminary characterization suggests that the neurotrophic factor in SCM appears to be a protein with a molecular weight greater than 8000 daltons.