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Quantitative freeze—fracture studies of membrane changes in chicken muscular dystrophy
Author(s) -
McLean Barbara,
MazenLynch Laila,
Shotton David M.,
McLean Gregor A.
Publication year - 1986
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880090605
Subject(s) - sarcolemma , caveolae , muscular dystrophy , myofibril , anatomy , latissimus dorsi muscle , biology , dystrophy , sarcoplasm , pathogenesis , chemistry , pathology , microbiology and biotechnology , membrane , medicine , skeletal muscle , endocrinology , biochemistry , genetics , endoplasmic reticulum
Muscular dystrophy induces extensive changes in the patterning of sarcolemmal caveolae of fast‐twitch fibers from the chicken posterior latissimus dorsi (PLD) muscle, which in healthy fibers are arranged in striking bands over the myofibrillar I‐bands. In dystrophic fibers the caveolae lack this patterned arrangement, and instead are dispersed over the entire sarcolemma, are irregular in shape, and are more numerous in older birds. Quantitative analysis of these differences provides three independent numerical indices of the dystrophic state and suggests that constraints responsible for normal patterning are lost in diseased fibers. These observations support theories that defects of the muscle plasma membrane are important for dystrophic pathogenesis in contrast, the sarcolemma of slow tonic fibers from anterior latissimus dorsi (ALD) and metapatagialis latissimus dorsi (MLD) muscles have randomly dispersed caveolae whose appearance and distribution are unaffected by the disease.