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Isolation and characterization of sarcoplasmic reticulum from normal and dystrophic chicken
Author(s) -
Kawamoto Richard M.,
Baskin Ronald J.
Publication year - 1986
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880090309
Subject(s) - endoplasmic reticulum , vesicle , microsome , oleic acid , chemistry , skeletal muscle , biochemistry , atpase , muscular dystrophy , fatty acid , biology , medicine , membrane , endocrinology , enzyme
Purified sarcoplasmic reticulum vesicles from normal and dystrophic chicken skeletal muscle have been isolated by a procedure employing pressure disruption of a microsomal suspension. The dystrophic sarcoplasmic reticulum (SR) exhibited reduced Ca+ + transport and phosphoenzyme formation, but the Ca + + ‐ATPase activity was normal. Normal and dystrophic SR showed similar lipid profiles, except for a significant increase in free fatty acids in the dystrophic SR. Investigations involving the interaction of oleic acid with normal SR showed fatty acids can induce conditions similar to those found in dystrophic SR.