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Localization of calcium‐activated neutral protease (CANP) in the peripheral nerve
Author(s) -
Kamakura Keiko,
Ishiura Shoichi,
Fujita Takehisa,
aka Ikuya,
Sugita Hideo
Publication year - 1985
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880080506
Subject(s) - endoneurium , ouchterlony double immunodiffusion , neurofilament , antiserum , axon , calcium , anatomy , myelin , biology , chemistry , peripheral nerve , endocrinology , medicine , immunohistochemistry , immunology , antibody , central nervous system
Localization of calcium‐activated neutral protease (CANP) in the rat peripheral nerve was studied by an indirect immunofluorescent method, using rabbit antiserum against CANP extracted from chicken skeletal muscle. Its specificity to CANP, as well as its cross‐reactivity with rat CANP, were confirmed by the Ouchterlony immunodiffusion procedure and immunoreplica method. CANP was demonstrated in the axon, but not in the myelin sheath or endoneurium. We could not conclude whether or not CANP in axons exists in association with neurofilaments.