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PC12 cells stimulate slow‐myosin light chain 2 synthesis in chick breast muscle culture
Author(s) -
Glass Charles A.,
Walker Charles,
Strohman Richard C.
Publication year - 1985
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880080505
Subject(s) - immunoglobulin light chain , myosin light chain kinase , myosin , neurite , acetylcholine , skeletal muscle , cell culture , in vitro , myocyte , microbiology and biotechnology , chemistry , biology , endocrinology , biochemistry , antibody , immunology , genetics
Skeletal muscle fibers developing in vitro synthesize predominantly fast‐myosin light chains, with a small contribution (less than 10%) from slow‐myosin light chain 2. Muscle fibers can be cocultured with a rat adrenal pheochromocytoma‐derived nerve cell line (PC12) known to display properties similar to sympathetic neurons. PC12 cells cultured alone synthesize catecholamines and respond to nerve growth factor by synthesizing acetylcholine and extending neurite structures. They also synthesize significant amounts of acetylcholine in the presence of nonneuronal cell types, including muscle. When cocultures of skeletal muscle fibers and PC12 cells are established, the muscle cells respond with an increased level of slow light chain 2 synthesis. Myosin light chains were identified by two‐dimensional gel electrophoresis and immunoblotting with an antiserum specific to slow light chain 2.