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The free cytoplasmic Ca 2+ levels in duchenne muscular dystrophy lymphocytes
Author(s) -
Klip Amira,
Elder Brian,
RuizFunes Hugo P.,
Buchwald Manuel,
Grinstein Sergio
Publication year - 1985
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880080408
Subject(s) - duchenne muscular dystrophy , cytoplasm , lymphoblast , muscular dystrophy , microbiology and biotechnology , chemistry , peripheral blood , endocrinology , medicine , biology , biochemistry , cell culture , genetics
An increased cellular Ca 2+ content has been associated with Duchenne muscular dystrophy (DMD). However, estimates of the free cytoplasmic Ca 2+ concentration ([Ca 2+ ]i) in cells of DMD patients were not available. We compared the [Ca 2+ ]i levels of normal and DMD peripheral blood lymphocytes and Epstein‐Barr virus‐transformed lymphoblasts using the novel probe, quin 2, an internally trapped fluorescent indicator. The [Ca 2+ ]i levels of normal and DMD cells were not significantly different.