Premium
Parvalbumin in cross‐reinnervated and denervated muscles
Author(s) -
Müntener Markus,
Berchtold Martin W.,
Heizmann Claus W.
Publication year - 1985
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880080209
Subject(s) - anatomy , electromyography , parvalbumin , medicine , denervation , neuroscience , physical medicine and rehabilitation , psychology
The extensor digitorum longus (EDL) muscle was cross‐reinnervated by the soleus (SOL) nerve, leading to the well‐known transformation toward a slow muscle. Nine weeks after the operation, the quantitative analysis of the Ca 2+ ‐binding protein, parvalbumin (PV), using highperformance liquid chromatography, showed a threefold reduction of PV in the cross‐reinnervated EDL muscle. Denervation of the EDL muscle, which leads to an increase of the half‐relaxation time, resulted in a 20% decrease of the PV concentration within 4 days. This significant lower PV level was detectable prior to any change of the myofibrillar adenosine triphosphatase (ATPase). Normal PV concentrations were reached after 9 weeks following self‐reinnervation of the EDL muscle. The experiments support the view that PV is involved in the relaxation of rat fast skeletal muscles and that its expression is dependent on nerve—muscle interaction. Since PV changes preceded histochemical changes after denervation, this protein may be a sensitive marker for early stages of neuromuscular disturbances.