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Neurons induce contractions in myotubes containing only muscular dysgenic nuclei
Author(s) -
Powell Jeanne A.,
Peterson Alan,
Paul Carol V.
Publication year - 1984
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880070304
Subject(s) - myogenesis , neuroscience , medicine , physical medicine and rehabilitation , biology , myocyte
Abstract Muscular dysgenic ( mdg / mdg ) myotubes cultured alone do not contract. Glucosephosphate isomerase (GPI‐1) isozymes were analyzed to determine the final genotype of cultured dysgenic ( mdg/mdg, gpi‐1 a/a ) myotubes to which normal embryonic spinal cord and limb cells (CBA/J +/+, gpi‐1 b/b ) had been added. Although both myoblast and spinal cord cell additions caused induction of spontaneous contractions, only myoblasts fused with the myotubes to create functional heterokaryons. Spinal cord cells did not fuse with dysgenic myotubes, but formed functional synapses.