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Effects of acrylamide and other sulfhydryl compounds in vivo and in vitro on staining of motor nerve terminals by the zinc iodide‐osmium technique
Author(s) -
Kemplay Sheila,
Cavanagh John B.
Publication year - 1984
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880070203
Subject(s) - acrylamide , staining , in vivo , chemistry , dithiothreitol , intraperitoneal injection , osmium , thiol , biochemistry , endocrinology , pathology , biology , medicine , polymer , organic chemistry , microbiology and biotechnology , copolymer , ruthenium , catalysis , enzyme
The zinc iodide‐osmium technique blackens motor nerve terminals by selectively staining synaptic vesicles. Intraperitoneal injections of acrylamide (30 mg/kg/day, 5 times each week) cause inhibition of staining by this technique so that approximately one third of the end‐plates in rat sternocostalis muscle are unstained after 24 hours, and by 17 days more than 70% are unstained. This is not associated with nerve fiber degeneration. A similar inhibition of staining can also be shown after prior incubation of the sternocostalis muscle in 4 m M acrylamide in oxygenated Ringer's solution. Intraperitoneal injection of the thiol group blocker N‐ethylmaleimide also causes marked inhibition of staining of motor end‐plates by this method. Dithiothreitol, which prevents the oxidation of thiol groups, will partly prevent the inhibition of staining by both acrylamide and N‐ethylmaleimide, when given in vivo.