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Double staining of the muscle fibers and interstitial tissue components for automatic analysis of muscle biopsies
Author(s) -
Hantaï Daniel,
Albe Xavier,
Fardeau Michel
Publication year - 1984
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880070202
Subject(s) - staining , adenosine triphosphatase , pathology , frozen section procedure , muscle tissue , muscle biopsy , ultraviolet light , anatomy , atpase , chemistry , biopsy , biology , medicine , biochemistry , enzyme , photochemistry
A staining technique is presented in order to facilitate the automatic analysis of muscle biopsies. It renders possible the visualization on the same section of the myofibrillary adenosine‐triphosphatase (ATPase) activity under the microscope with transmitted light, and of the fluorescent staining of fibronectin which marks the outlines of the muscle fibers by a simple switch to ultraviolet (UV) light.