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Quantitation of metabolites in human skeletal muscle during rest and exercise: A comparison of methods
Author(s) -
Sabina Richard L.,
Swain Judith L.,
Bradley Walter G.,
Holmes Edward W.
Publication year - 1984
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880070113
Subject(s) - metabolite , creatine , normalization (sociology) , coefficient of variation , human muscle , skeletal muscle , chemistry , vastus lateralis muscle , nad+ kinase , medicine , endocrinology , biochemistry , chromatography , sociology , anthropology , enzyme
Quantitation of muscle metabolites in vastus lateralis needle biopsies from human subjects at rest and following exercise is evaluated using three different methods of normalization of the data, i.e., total protein, total creatine, and NAD + . Coefficients of variation between patient analyses are similar when the metabolite data are normalized by any of the descriptors in resting muscle and in muscle samples obtained following vigorous exercise. When multiple samples are taken from individual patients the intrapatient coefficient of variation is less than that for interpatient comparisons. Normalization to total creatine or NAD + yields significantly smaller coefficients of variation than does normalization to total protein in these intrapatient comparisons ( P < 0.05). Based on these comparisons, we conclude that any of these methods evaluated for normalizing metabolite content is adequate for studying human subjects, but the total creatine and NAD + methods may offer some advantage because of the lower variability obtained with these analyses.