Premium
Immunohistochemical demonstration of β‐enolase in human skeletal muscle
Author(s) -
Ibi Tohru,
Sahashi Ko,
Kato Kanefusa,
Takahashi Akira,
Sobue Itsuro
Publication year - 1983
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880060907
Subject(s) - immunohistochemistry , enolase , skeletal muscle , pathology , medicine , anatomy
We have localized β‐enolase activity in human skeletal muscle fiber using the immunohistochemical method (two‐step method). The first immunoreagent was rabbit anti‐human β‐enolase serum raised in New Zealand white rabbit, and the second was peroxidase conjugated staphylococcal protein A. The immunohistochemical reaction for β‐enolase was noted higher in type 2 fiber, which demonstrates the low oxidative and high glycolytic enzyme activity, than in type 1 fiber.