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Effects of insulin on protein synthesis in muscles from normal and dystrophic mice
Author(s) -
Ballard F. John,
Nield Michelle K.,
Tomas Frank M.
Publication year - 1983
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880060709
Subject(s) - medicine , endocrinology , insulin , anabolism , protein metabolism , muscular dystrophy , soleus muscle , in vitro , muscle protein , metabolism , biology , chemistry , skeletal muscle , biochemistry
Protein synthesis in soleus and extensor digitorum logus (EDL) muscles was measured in vitro to test the hypothesis that the lack of muscle protein accumulation in dystrophic conditions could be caused by a reduced sensitivity to insulin. We demonstrate that physiological insulin concentrations stimulate protein synthesis in soleus muscles from normal mice but not from muscles obtained from dystrophic ( dy ) animals. The difference is lost at very high insulin concentrations (1 μM) and could not be shown at any concentration with EDL muscles. These results, together with the reported reduced inhibitory effect of insulin on protein synthesis in dystrophic hamsters and on protein breakdown in dystrophic mice, suggest, that protein metabolism in certain muscles from dystrophic animals may be less responsive to the anabolic effects of insulin.