Premium
Skeletal muscle lysosomes: Comparison of lysosomes from normal and dystrophic avian pectoralis muscle as a function of age
Author(s) -
Baxter Jeffrey H.,
Suelter Clarence H.
Publication year - 1983
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880060304
Subject(s) - pectoralis major muscle , pectoralis muscle , skeletal muscle , function (biology) , anatomy , biology , microbiology and biotechnology
The properties of skeletal muscle lysosomes from normal and dystrophic chickens were studied to assess their involvement in the dystrophic process. A method is described for isolation of a three‐to‐sevenfold purified lysosome fraction with 29–33% yield. Lysosomal enzymes in crude homogenates and isolated lysosome‐enriched fractions from dystrophic muscle exhibit decreased latency for N ‐acetyl β‐ D ‐glucosaminidase, acid phosphatase, and cathepsin D. However, no differences in the fragility of lysosomes in isolated lysosome‐enriched fractions from normal and dystrophic muscle were observed using shear, sonication and detergent stress. Lower percent recovery, enrichment factor and percent latency of acid phosphatase compared to N ‐acetyl‐β‐ D ‐glucosaminidase and cathepsin D were observed from both normal and dystrophic muscle. These results are consistent with the presence of a significant amount of nonlysosomal acid phosphatase activity in skeletal muscle.