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Stereometric estimation of the area of the freeze‐fractured membrane
Author(s) -
Engel Andrew G.,
Fukunaga Hidetoshi,
Osame Mitsuhiro
Publication year - 1982
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880050904
Subject(s) - plotter , point (geometry) , stereoscopy , surface (topology) , computer graphics (images) , matching (statistics) , optics , geometry , materials science , computer vision , mathematics , physics , computer science , statistics
Abstract Stereo‐pair micrographs of freeze‐fracture replicas taken at +6 and −6 degrees and enlarged 55,000 diameters were precisely aligned on a digitizing pad linked to a plotter and programmable calculator. A point grid spaced at 0.23 μm was placed over the left photo‐pair. Under stereoscopic observation, matching points were placed by hand on the right photo‐pair for every point in the applied grid that fell over the membrane surface of interest in the left photo‐pair. The x, y coordinates of every point in the two photos were read with the digitizer. These data allowed the calculation of the X, Y, Z coordinates of all points in the untilted object space, 3‐D reconstruction of the analyzed surface, and estimation of the membrane surface area.