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Palmitate oxidation in human muscle: Comparison to CPT and carnitine
Author(s) -
Shumate Jack B.,
Carroll James E.,
Brooke Michael H.,
Choksi Rati M.
Publication year - 1982
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880050309
Subject(s) - carnitine , muscular dystrophy , myopathy , human muscle , duchenne muscular dystrophy , endocrinology , medicine , beta oxidation , citrate synthase , denervation , acetylcarnitine , metabolism , chemistry , biochemistry , biology , skeletal muscle , enzyme
The evaluation of palmitate oxidation in muscle tissue may be a useful screening test for detecting defects in fatty acid metabolism in human neuromuscular disease. If the test is to be useful, it is necessary to obtain data on a wide variety of muscle illnesses for comparative purposes. We report our experience with palmitate oxidation, muscle carnitine, and carnitine palmityl transferase (CPT) activity in 148 muscle biopsies from a variety of illnesses. The efficacy of using total protein, citrate synthase, and (1‐ 14 C) pyruvate oxidation as internal references was investigated. Palmitate oxidation was significantly less than normal ( P ≤ 0.01) in Duchenne muscular dystrophy, congenital nonprogressive myopathy, congenital muscular dystrophy, malignant hyperpyrexia, and denervation, depending on the internal reference used. Muscle carnitine levels followed a similar pattern, however, CPT activity did not. The possibility of these findings being secondary to inactivity is discussed.