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Vital‐freezing of human muscle cultures for storage and reculture
Author(s) -
Carter Linda S.,
Askanas Valerie
Publication year - 1981
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880040503
Subject(s) - fastidious organism , liquid nitrogen , human muscle , tissue culture , myocyte , liquid culture , biology , anatomy , chemistry , microbiology and biotechnology , skeletal muscle , biochemistry , in vitro , bacteria , genetics , botany , organic chemistry
The vital‐freezing of human muscle cultures enabling long‐term storage in liquid nitrogen and future reculturing is described. Although human muscle is more fastidious in tissue culture than most cells of other origin, it was successfully frozen and re‐established in culture. Cultures re‐established after storage in liquid nitrogen differentiated and matured in the same way as primary human muscle cultures, and had the same histochemical and electron microsopic characteristics.