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Membrane elasticity of erythrocytes from normal and dystrophic mice
Author(s) -
Missirlis Yannis F.,
Vanderwel Marianne,
Brain Michael C.
Publication year - 1981
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880040211
Subject(s) - membrane , shear stress , erythrocyte membrane , strain (injury) , chemistry , elasticity (physics) , red blood cell , biophysics , anatomy , physics , biology , mechanics , biochemistry , thermodynamics
The membrane deformability of erythrocytes from normal and dystrophic mice was determined using a flow channel technique whereby erythrocytes attached to the floor of a parallel plate channel were deformed by fluid shear forces. A nonlinear stress‐strain experimental behavior was observed for both populations of erythrocytes which was best described with a polynominal expression: τ S = aε x + [bε x 3 /(2ε x + 1)]. A comprehensive statistical analysis of the data indicated that a large percentage of the variance of the data was due to the experimental design. Furthermore, the 2 populations of cells were different in terms of the strain‐stress relationship which best fitted the data, i.e., ε x = ατ s + βτ s 2 + γτ s 3 . Up to a shear stress of 5.5 dyn/cm 2 , where 95% of the data points were found, the dystrophic erythrocytes were slightly but significantly more deformable than the normal erythrocytes.