Premium
Normal and dystrophic embryonic chicken pectoralis muscle cultures: III. Viral infection of normal cell cultures
Author(s) -
McConnell David G.,
Gardner M. Elizabeth,
Tuomari Anne V.,
Young Ronald B.,
Suelter Clarence H.
Publication year - 1981
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880040209
Subject(s) - leucosis , embryo , biology , virus , fibroblast , pectoralis muscle , embryonic stem cell , cell culture , virology , myocyte , andrology , cell , tissue culture , pathology , anatomy , endocrinology , microbiology and biotechnology , biochemistry , medicine , genetics , in vitro , gene
Twelve‐day normal and dystrophic chick embryo breast muscle cells were cultured for up to 14 days, using normal embryo extract in the culture medium. Fluorodeoxyuridine was added on day 3 to suppress fibroblast overgrowth. Three of 6 experiments with normal cells were severely infected with avian leucosis/sarcoma (ALS) virus particles. The findings appear to lend support to the suggestion that the ALS virus is a mitochondriophage. Infected cultures demonstrated a depressed rate of total protein synthesis as reflected by incorporation of [ 3 H]leucine. Extractable protein and total protein content were also depressed within several days after ALS particles were identified. These findings reinforce caution in acceptance of the common assumption that viral infections are an unavoidable and metabolically benign component of muscle cell cultures.