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Normal and dystrophic embryonic chicken pectoralis muscle cultures: I. Cell differentiation, protein synthesis, and enzyme levels
Author(s) -
Young Ronald B.,
McConnell David G.,
Suelter Clarence H.,
Phillips Teresa A.
Publication year - 1981
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880040207
Subject(s) - myogenesis , biology , multinucleate , myocyte , creatine kinase , myofibril , myosin , endocrinology , medicine , pectoralis muscle , cell culture , muscular dystrophy , biochemistry , microbiology and biotechnology , anatomy , genetics
Normal (line 200) and dystrophic (line 307) embryonic chicken pectoralis muscle cells were studied in cell culture over a period of 2 weeks. During the first 4 days, normal and dystrophic cultures exhibited similar developmental increases in the number of nuclei within multinucleated myotubes; however, dystrophic muscle cells degenerated approximately twice as fast as normal cells once the initial burst of myoblast fusion was complete. The apparent synthesis rate of nonmyofibrillar proteins was similar in normal and dystrophic cells throughout development, but the apparent synthesis rates of myosin heavy chain and the myofibrillar protein fraction were 50%–90% higher in dystrophic muscle cultures once maturity had been reached (days 6–14). The specific activities of creatine kinase and phosphofructokinase were not affected by the dystrophic condition; however, specific activity of AMP‐deaminase was depressed 25%–40% in the dystrophic muscle cultures.