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Ca‐activated neutral protease and its inhibitors: In vitro effect on intact myofibrils
Author(s) -
Sugita Hideo,
Ishiura Shoichi,
Suzuki Koichi,
Imahori Kazutomo
Publication year - 1980
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.880030410
Subject(s) - myofibril , leupeptin , myosin , protease , chemistry , biochemistry , in vitro , troponin , troponin i , neutral protease , microbiology and biotechnology , enzyme , biology , medicine , myocardial infarction
SDS gel electrophoresis of troponin and myosin extracted from chicken myofibrils pretreated with Ca‐activated neutral protease showed a pattern similar to that observed in the muscles of patients with Duchenne muscular dystrophy, that is, a decrease in troponin‐l and troponin‐C with relative preservation of troponin‐T and degradation of the heavy chain of myosin. Also, α‐actinin and troponin‐C were released from myofibrils. Two inhibitors of Ca‐activated neutral protease, leupeptin and E‐64, inhibited the degradative action of Ca‐activated neutral protease on intact myofibrils in vitro.