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One‐hour universal protocol for mouse genotyping
Author(s) -
Joubert Romain,
Mariot Virginie,
Dumonceaux Julie
Publication year - 2020
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.26841
Subject(s) - genotyping , protocol (science) , sexing , computational biology , biology , polymerase chain reaction , genetics , computer science , genotype , medicine , gene , pathology , alternative medicine
Background Transgenic animals are widely used for research and for most of them, genotyping is unavoidable. Published protocols may be powerful but may also present disadvantages such as their cost or the requirement of additional steps/equipment. Moreover, if more than one strain must be genotyped, several protocols may need to be developed. Methods we adapted the existing amplification‐resistant mutation protocol to develop the 1‐h universal genotyping protocol (1‐HUG), which allows the robust genotyping of genetically modified mice in 1 h from sample isolation to polymerase chain reaction gel running. Results This protocol allows the genotyping of different mouse models including mdx mouse, and FLExDUX4 and HSA‐MerCreMer alone or in combination. It can be applied to different types of genomic modifications and to sexing. Conclusions The 1‐HUG protocol can be used routinely in any laboratory using mouse models for neuromuscular diseases.