Premium
Altered muscle electrical tissue properties in a mouse model of premature aging
Author(s) -
ClarkMatott Joanne,
Nagy Janice A.,
Sanchez Benjamin,
Taylor Rebecca,
Riveros Daniela,
Abraham Neeta A.,
Simon David K.,
Rutkove Seward B.
Publication year - 2019
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.26714
Subject(s) - nicotinamide mononucleotide , myocyte , nicotinamide , medicine , endocrinology , chemistry , biology , biophysics , nad+ kinase , biochemistry , nicotinamide adenine dinucleotide , enzyme
Improved methods are needed to detect and quantify age‐related muscle change. In this study we assessed the electrical properties of muscle impacted by acquired mitochondrial DNA mutations via the PolG mouse, which exhibits typical age‐associated features, and the impact of a potential therapy, nicotinamide mononucleotide (NMN). Methods The gastrocnemii of 24 PolG and 30 wild‐type (WT) mice (8 PolG and 17 WT treated with NMN) were studied in an electrical impedance‐measuring cell. Conductivity and relative permittivity were determined from the impedance data. Myofiber cross‐sectional area (CSA) was quantified histologically. Results Untreated PolG mice demonstrated alterations in several impedance features, including 50‐kHz relative permittivity and center frequency. A potential effect of NMN was also observed in these parameters in PolG but not WT animals. Impedance values correlated with myofiber CSA. Discussion Electrical impedance is sensitive to myofiber features considered characteristic of aging and to the impact of a potential therapy.