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Frequency‐dependent lipid raft uptake at rat diaphragm muscle axon terminals
Author(s) -
Gonzalez Porras Maria A.,
Fogarty Matthew J.,
Gransee Heather M.,
Sieck Gary C.,
Mantilla Carlos B.
Publication year - 2019
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.26421
Subject(s) - axon , lipid raft , stimulation , raft , diaphragm (acoustics) , axon terminal , biophysics , motor nerve , phrenic nerve , ganglioside , neuromuscular junction , cholera toxin , chemistry , biology , microbiology and biotechnology , membrane , anatomy , endocrinology , neuroscience , biochemistry , respiratory system , physics , organic chemistry , acoustics , loudspeaker , copolymer , polymer
: In motor neurons, cholera toxin B (CTB) binds to the cell‐surface ganglioside GM1 and is internalized and transported via structurally unique components of plasma membranes (lipid rafts). Methods : Lipid raft uptake by axon terminals adjoining type‐identified rat diaphragm muscle fibers was investigated using CTB and confocal imaging. Results : Lipid raft uptake increased significantly at higher frequency stimulation (80 Hz), compared with lower frequency (20 Hz) and unstimulated (0 Hz) conditions. The fraction of axon terminal occupied by CTB was ∼45% at 0‐ or 20‐Hz stimulation, and increased to ∼65% at 80 Hz. Total CTB fluorescence intensity also increased (∼20%) after 80‐Hz stimulation compared with 0 Hz. Discussion : Evidence of increased lipid raft uptake at high stimulation frequencies supports an important role for lipid raft signaling at rat diaphragm muscle axon terminals, primarily for motor units physiologically activated at the higher frequencies. Muscle Nerve 59:611–611, 2019