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Effects of mesenchymal stromal cells on type 1 diabetes mellitus rat muscles
Author(s) -
Sabadine Maria Augusta,
Russo Thiago Luiz,
Luna Genoveva Flores,
Oliveira Leal Angela Merice
Publication year - 2018
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.26196
Subject(s) - muscle atrophy , medicine , transplantation , endocrinology , mesenchymal stem cell , fibrosis , atrophy , streptozotocin , skeletal muscle , myostatin , diabetes mellitus , pathology
: Type 1 diabetes mellitus (DM) causes marked skeletal muscle atrophy. Mesenchymal stromal cells (MSC) are an attractive therapy to avoid diabetic complications because of their ability to modify the microenvironment at sites of tissue injury. The objective of this study was to evaluate the effects of MSC transplantation on muscle adaptation caused by diabetes. Methods : DM was induced by streptozotocin (STZ), and the diabetic animals received systemic MSC transplantation. The von Frey test and footprint analysis were used to assess sensation and sensory motor performance, respectively. Tibialis anterior muscles were investigated by morphology; molecular markers atrogin‐1/muscle RING‐finger protein‐1, nuclear factor κB/p38 mitogen‐activated protein kinase, tumor necrosis‐like weak inducer of apoptosis/fibroblast growth factor‐inducible 14, myostatin, myogenic differentiation 1, and insulin‐like growth factor 1 were also assessed. Results : MSC transplantation improved sensation and walking performance and also decreased muscle fibrosis in DM rats by modulating atrogenes but did not prevent muscle atrophy. Discussion : MSCs can reduce muscle and functional complications that result from type 1 DM in rats. Muscle Nerve 58 : 583–591, 2018