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Label‐free identification of myopathological features with coherent anti‐Stokes Raman scattering
Author(s) -
Niedieker Daniel,
GrosserÜschkamp Frederik,
Schreiner Anja,
Barkovits Katalin,
Kötting Carsten,
Marcus Katrin,
Gerwert Klaus,
Vorgerd Matthias
Publication year - 2018
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.26140
Subject(s) - raman scattering , staining , raman spectroscopy , silver stain , pathology , chemistry , medicine , biology , optics , physics
: The aim of this study was the label‐free identification of distinct myopathological features with coherent anti‐Stokes Raman scattering (CARS) imaging, which leaves the sample intact for further analysis. Methods : The protein distribution was determined without labels by CARS at 2,930 cm −1 and was compared with the results of standard histological staining. Results : CARS imaging allowed the visualization of glycogen accumulation in glycogen storage disease type 5 (McArdle disease) and of internal nuclei in centronuclear myopathy. CARS identified an inhomogeneous protein distribution within muscle fibers in sporadic inclusion body myositis that was not shown with standard staining. In Duchenne muscular dystrophy, evidence for a higher protein content at the border of hypercontracted fibers was detected. Discussion : CARS enables the label‐free identification of distinct myopathological features, possibly paving the way for subsequent proteomic, metabolic, and genomic analyses. Muscle Nerve 58 : 457–460, 2018